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Updated: Aug 23, 2026

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
Transient species in the stepwise interconversion of NADH and NAD+
Jerzy Gebicki1, Andrzej Marcinek, Jacek Zielonka
1Institute of Applied Radiation Chemistry, Technical University, 90-924 Lodz, Poland.
Abstract:
Two mechanisms of the interconversion of NADH and NAD+ in the coenzyme itself and in its analogues are discussed: a one-step hydride transfer and a stepwise electron-proton-electron transfer. Direct characterization of the transient species in the stepwise process and inversion of the stability order of the keto and enol tautomers is presented. The nonenzymatic and enzyme-mediated reactions of selected pyridinium salts that affect the NADH <==>NAD+ equilibrium are discussed in terms of their potential cytotoxicity.
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