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[Experimental study on islet cells in rats under condition of three-dimensional microgravity]
Chun Song1, Xiu-qing Duan, Yi Zhou
1The Key Laboratory of Cell Transplantation of Ministry of Health, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
Objective:
To study the survival rate and secretory function of islet cells in rats under condition of three-dimensional microgravity.
Methods:
Isolated islet cells were assigned to flask-cultured or bioreactor-cultured. Survival rate of islets cultured for days 3, 7, 14 in stationary flasks or microgravity bioreactors was measured by AO-PI double-staining. Cultured islets were identified by dithizone (DTZ) staining, and insulin contents of different culture liquids were measured by radioimmunoassay.
Results:
Pancreatic islets stained nacarat with DTZ were easily visualized. When islet cells were cultured for 7 days and 14 days, survival rate of bioreactor-cultured islets was (0.9000 +/- 0.0107)% and 0.8038% +/- 0.0092% and higher than flask-cultured islets (P < 0.01). Insulin level of bioreactor-cultured islets is (70.875 +/- 0.31) m micro /L on the cultured 7 days while flask-cultured islets is (41.246 +/- 0.35) m micro /L. There was statistically significant difference of insulin production between the two groups (P < 0.01). Bioreactor-cultured islet contents were higher than flask-cultured on the cultured 14, 21 and 30 days (P < 0.01).
Conclusions:
Islet cells survival rate and secretory function revealed that bioreactor-cultured islets functioned better compared with flask-cultured islets.