Clinical application of laser-assisted ICSI: a pilot study

Human oocyte survival after intracytoplasmic sperm injection (ICSI) can be compromised by abnormal oolemma breakage patterns during the penetration of the microinjection needle. We previously reported a case of repeated ICSI failures associated with abnormal oolemma breakage in which a substantial improvement and successful pregnancy were achieved by performing ICSI through a laser-drilled hole in the zona pellucida (laser-assisted ICSI). This study was undertaken to compare the efficacy of laser-assisted ICSI with that of conventional ICSI in patients whose oocytes present this particular feature. Oocytes treated by laser-assisted ICSI (n=140) survived better (97.9% versus 85.7%; P<0.05) and tended to form more two-pronucleated zygotes (78.6% versus 69.2%; P=0.07) and less zygotes with three or more pronuclei (2.8% versus 7.8%; P=0.06) as compared with sibling oocytes treated by conventional ICSI (n=140). These data show that laser-assisted ICSI is suitable for oocytes with propensity for sudden oolemma breakage during conventional ICSI. The reduction of oocyte damage with laser-assisted ICSI as compared to conventional ICSI may be useful in other clinical indications where only few oocytes are available or when the oocyte survival after ICSI can be supposed to be compromised.