Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An effective morphometric method for electron microscopic studies on papillary muscles.

G Mall, H Reinhard, K Kayser

    Virchows Archiv. A, Pathological Anatomy and Histology
    |September 7, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Sensitivity of temperature-based time since death estimation on measurement location.

    International journal of legal medicine·2023
    Same author

    Delayed occurrence of traumatic aortic dissection? Biomechanical considerations and literature.

    International journal of legal medicine·2022
    Same author

    [Bridging vein injuries in shaken baby syndrome : Forensic-radiological meta-analysis with special focus on the tadpole sign].

    Der Radiologe·2020
    Same author

    Slip and tilt: modeling falls over railings.

    International journal of legal medicine·2020
    Same author

    Forensic postmortem computed tomography in suspected unnatural adult deaths.

    European journal of radiology·2020
    Same author

    Injury pattern and the biomechanical assessment of skull fracture risk in blows with a rubber mallet.

    Forensic science international·2020

    This study quantifies subcellular structures in rat papillary muscles using morphometry. The methods are validated for experimental use, providing reliable data on myocardial cell components.

    Area of Science:

    • Cardiovascular Biology
    • Cell Biology
    • Histology

    Background:

    • The left ventricular posterior papillary muscles are crucial for cardiac function.
    • Understanding their ultrastructure is vital for studying cardiac diseases.
    • Quantitative morphometry provides precise insights into myocardial tissue composition.

    Purpose of the Study:

    • To perform detailed morphometric analysis of rat left ventricular posterior papillary muscles.
    • To quantify volume and surface densities of key cellular and extracellular components.
    • To establish the reliability of the morphometric method for experimental research.

    Main Methods:

    • Morphometric analysis of myocardial tissue from Wistar rats.
    • Calculation of volume densities for cellular and interstitial components.

    Related Experiment Videos

  • Calculation of surface densities for cellular membranes and tubules.
  • Specific sectioning angle (32.4 degrees) determined by Whitehouse's equation (1974).

    • Main Results:

    • Detailed quantitative data on volume densities of myocardial cells, interstitial space, nuclei, sarcoplasm, mitochondria, myofibrils, ground substance, and T-tubules.
    • Quantitative data on surface densities of myocardial cells, mitochondrial membranes, and T-tubules.
    • Low standard errors indicate method adequacy despite single section analysis.

    Conclusions:

    • The described morphometric technique is reliable and adequate for experimental studies on myocardial ultrastructure.
    • The study provides a quantitative basis for future research into papillary muscle biology and pathology.
    • Correction procedures for oblique sectioning artifacts are discussed, enhancing methodological rigor.