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Updated: Aug 14, 2026

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
Published on: September 13, 2013
In vitro semisynthesis and applications of C-terminally modified rab proteins
Thomas Durek1, Roger S Goody, Kirill Alexandrov
1Max-Planck-Institut für Molekulare Physiologie, Dortmund, Germany.
Abstract:
Expressed protein ligation is a powerful tool for the generation of natively folded proteins composed of recombinantly generated and chemically synthesized polypeptides. Using this approach, we developed protocols for the production of prenylated and/or otherwise-labeled Rab GTPase. The protocols are generally applicable to most small GTPases that can be supplied with a variety of new chemical functionalities. We used semisynthetic fluorescently labeled Rab7 GTPase as a molecular probe to study protein-protein interactions with components of the prenylation machinery
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