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Updated: Aug 23, 2026

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening
Published on: May 13, 2020
Generation and characterization of ras lipoproteins based on chemical coupling
Melanie Wagner1, Jürgen Kuhlmann
1Max-Planck-Institut für Molekulare Physiologie, Dortmund, Germany.
Abstract:
Chemically synthesized truncated Ras proteins are coupled to C-terminal Ras peptides via a maleimidocaproyl linker. The resulting product is isolated by extraction with Triton X-114. The biological activity of these oncogenic Ras lipoproteins can be determined in a cell-based differentiation assay by microinjection into PC12 cells.
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