Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thermophilic bacterial DNA polymerases with reverse-transcriptase activity.

Harini Shandilya1, Kate Griffiths, Elizabeth K Flynn

  • 1Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, CA 92008, USA.

Extremophiles : Life Under Extreme Conditions
|June 16, 2004
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Image-based quantitative osteotomy planning for congenital proximal radioulnar synostosis: plan-execution discrepancy and postoperative radiographic outcomes.

Journal of shoulder and elbow surgery·2026
Same author

Native trees are related to advanced bird breeding phenology and increased reproductive success along an urban gradient.

Ecology·2026
Same author

Disruption of Bacterial Biofilms by Photothermal Energy to Overcome Antibiotic Resistant Phenotype.

Military medicine·2026
Same author

Sequelae of Infantile Elbow Infection in an Adolescent: A Case Report with 3D-Printed Assisted Preoperative Planning.

JBJS case connector·2025
Same author

Thermosensitive hydrogel for enhanced burn wound healing: Oxygen and exosome co-delivery to overcome hypoxia-induced barriers.

International journal of biological macromolecules·2025
Same author

Derivation of AZD5335, a Novel FRα-Targeted TOP1i-Loaded ADC, for the Treatment of FRα-Expressing Cancers.

Clinical cancer research : an official journal of the American Association for Cancer Research·2025

Researchers identified bacterial DNA polymerase I (polI) genes using PCR and characterized nine enzymes. Several polI polymerases exhibited significant reverse-transcriptase activity, particularly those from thermophilic bacteria.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genomics

Background:

  • Bacterial DNA polymerase I (polI) enzymes are crucial for DNA replication and repair.
  • Identifying and characterizing novel polI enzymes from diverse bacterial sources can reveal new enzymatic properties.

Purpose of the Study:

  • To develop a method for rapid identification and isolation of polI genes from various bacterial divisions.
  • To characterize the biochemical properties of purified recombinant polI enzymes, focusing on their polymerase and potential reverse-transcriptase activities.

Main Methods:

  • Identification of conserved motifs in known bacterial polI DNA polymerase sequences.
  • Design of degenerate PCR primers for polI gene amplification.
  • PCR amplification and isolation of 13 polI genes from thermophilic bacteria.

Related Experiment Videos

  • Purification and biochemical characterization of nine recombinant polI enzymes.

    • Main Results:

    • Successfully identified and isolated 13 polI genes from a diverse range of thermophilic bacteria.
    • Characterized nine purified recombinant polI enzymes.
    • Observed significant reverse-transcriptase activity in several polI enzymes, especially those from *Bacillus caldolyticus* EA1, *Caldibacillus cellovorans* CompA.2, and *Clostridium stercorarium* in the presence of Mg2+.

    Conclusions:

    • The developed PCR-based method enables rapid identification and isolation of polI genes across bacterial divisions.
    • Several novel thermophilic bacterial polI polymerases possess significant reverse-transcriptase activity, suggesting potential applications in molecular biology.