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Updated: Aug 12, 2026

In situ Protocol for Butterfly Pupal Wings Using Riboprobes
Published on: May 28, 2007
Alterations in ecdysone content during the post-embryonic development of Chironomus thummi: correlations with
Abstract:
The ecdysone titer of larvae and pupae of Chironomus thummi was determined by radioimmune assay (RIA) and revealed a concentration of about 150 ng/g fresh weigth in late third instar larvae and a peak of more than 450 ng/g fresh weight just preceding pupation. The ecdysone titer curve shows a high degree of correlation with previously observed puffing activity at the ecdysone sensitive chromosomal site IIIdl. Further, beta-ecdysone is the only endogenous ecdysone detected by high pressure liquid chromatography during larval-pupal development. It was also observed that developmentally arrested Chironomus contain less ecdysone than "normal" larvae.
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