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Related Experiment Videos

Human challenge pilot study with Cyclospora cayetanensis.

Edith M Alfano-Sobsey1, Mark L Eberhard, John R Seed

  • 1Office of Public Health Preparedness and Response, Raleigh, North Carolina, USA. ealfano@ph.co.durham.nc.us

Emerging Infectious Diseases
|June 18, 2004
PubMed
Summary
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This pilot study found that ingesting Cyclospora cayetanensis oocysts did not cause illness in healthy volunteers. No symptoms or oocyst detection occurred during 16 weeks of monitoring, suggesting low infectivity at the tested doses.

Area of Science:

  • * Infectious Diseases
  • * Parasitology
  • * Gastroenterology

Background:

  • * Cyclospora cayetanensis is an emerging foodborne pathogen causing cyclosporiasis.
  • * Human infection typically results from ingesting contaminated food or water.
  • * Understanding infective dose is crucial for public health risk assessment.

Purpose of the Study:

  • * To determine the infectivity of Cyclospora cayetanensis in humans.
  • * To establish a potential human infectious dose for Cyclospora.

Main Methods:

  • * A pilot study involving seven healthy human volunteers.
  • * Volunteers ingested varying doses of Cyclospora oocysts (200-49,000).
  • * Monitoring for 16 weeks included symptom assessment and stool sample analysis.

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Main Results:

  • * None of the seven volunteers developed symptoms of gastroenteritis.
  • * No Cyclospora oocysts were detected in any stool samples throughout the study.
  • * The study suggests a high threshold for Cyclospora infection in healthy adults.

Conclusions:

  • * Ingestion of up to 49,000 Cyclospora oocysts did not lead to infection or illness in healthy volunteers.
  • * Further research is needed to define the precise human infectious dose.
  • * Findings contribute to understanding the epidemiology and transmission of cyclosporiasis.