Related Experiment Video

Updated: Aug 23, 2026

Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds
07:13

Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds

Published on: August 8, 2025

[Study on the molecular mechanism of quinolone resistance in Shigellae spp]

Jing-yuan Zhu1, Guang-cai Duan, Yuan-lin Xi

  • 1The Department of Environmental Hygiene, College of Public Health, Zhengzhou University, Zhengzhou 450052, China.

Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi
|June 18, 2004
PubMed
Abstract

Insights

Shigella species exhibit high resistance to quinolones, primarily due to mutations in the gyrA gene, and secondarily in the parC gene. This study investigated the resistance mechanisms in clinical Shigella isolates.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Pharmacology

Context:

  • Quinolone antibiotics are crucial for treating bacterial infections.
  • Shigella species are significant human pathogens.
  • Understanding antimicrobial resistance is vital for public health.

Purpose:

  • To investigate the quinolone resistance and its underlying mechanisms in Shigella species.
  • To determine the prevalence of mutations in gyrA and parC genes associated with quinolone resistance.

Summary:

  • This study analyzed 73 clinical Shigella isolates for their susceptibility to pipemidic acid, ciprofloxacin, norfloxacin, and ofloxacin.
  • High resistance rates were observed. Mutations in gyrA were found in 91% of resistant isolates, with parC mutations in 7.5%.

Impact:

  • The findings highlight the significant role of target gene mutations, particularly in gyrA, in conferring quinolone resistance in Shigella.
  • This research provides critical insights for developing effective treatment strategies against Shigella infections.

More Related Videos

Epithelial Cell Infection Analyses with Shigella04:56

Epithelial Cell Infection Analyses with Shigella

Published on: February 9, 2024

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes08:23

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Published on: March 2, 2020

Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
Inhibitors of Bacterial DNA Synthesis01:28

Inhibitors of Bacterial DNA Synthesis

Bacterial pathogens depend on precise and efficient DNA replication to sustain infection. Two type II topoisomerases—DNA gyrase and topoisomerase IV—are critical to this process, as they resolve DNA supercoiling and unlink chromosomes during replication. Fluoroquinolones, synthetic derivatives of quinolones, exploit this mechanism by stabilizing the transient DNA–enzyme cleavage complex, preventing strand religation, and causing lethal double-strand breaks. These antibiotics are selectively...
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...