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Mcm10 and Cdc45 cooperate in origin activation in Saccharomyces cerevisiae

Sara L Sawyer1, Irene H Cheng, Weihang Chai

  • 1Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.

Journal of Molecular Biology
|June 18, 2004
PubMed

Insights

The Mcm10 protein is essential for recruiting the Cdc45 protein to DNA replication origins in yeast. This interaction is critical for initiating DNA replication and cell growth.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Mcm10 plays a vital role in eukaryotic DNA replication initiation.
  • Understanding Mcm10's precise function in assembling replication factors is crucial.

Purpose of the Study:

  • To investigate the role of Mcm10 in the assembly of initiation factors at the ARS1 replication origin in yeast.
  • To elucidate the mechanism by which Mcm10 facilitates the recruitment of Cdc45.

Main Methods:

  • Analysis of the mcm10-1 mutant yeast strain.
  • Assessing the association of pre-replication complex (pre-RC) components (Cdc6, Mcm7, Cdc45) with the ARS1 origin.
  • Investigating the physical interaction between Mcm10 and Cdc45.

Main Results:

  • The pre-RC components Cdc6 and Mcm7 successfully associate with ARS1 in the mcm10-1 mutant.
  • Cdc45 association with ARS1 is significantly reduced in the mcm10-1 mutant.
  • Overexpression of Mcm10-1 or Cdc45 rescues the growth defect of the mcm10-1 mutant.
  • A physical interaction between Mcm10 and Cdc45 is disrupted in the mcm10-1 mutant.

Conclusions:

  • Mcm10 is specifically required for recruiting Cdc45 to the pre-RC at replication origins.
  • The interaction between Mcm10 and Cdc45 is essential for efficient Cdc45 loading onto ARS1.
  • This interaction facilitates the initiation of DNA replication.

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