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Updated: Aug 23, 2026

Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram
Published on: November 27, 2016
[Content determination of S-citalopram by chiral high-performance liquid chromatography]
Xue-mei Yang1, Xu Liu, Yi-chen Yan
1Department of Chemistry, First Military Medical University, Guangzhou 510515, China.
Objective:
To separate the enantiomers of citalopram and determine the content of S-citalopram using chiral high-performance liquid chromatography (HPLC).
Methods:
The chiral column CHIROBIOTIC V was used with the mobile phase using methanol-acetic acid-triethylamine (100:0.1:0.1) at the detection wavelength of 240 nm, column temperature of 20 degrees Celsius and flow rate of 1.0 ml/min.
Results:
Complete separation of the enantiomers of citalopram was achieved, and S-citalopram exhibited good linearity within the concentration range of 10 to 150 microg/ml (r=0.9991, n=5).
Conclusion:
This method allows accurate quantification of S- and R-citalopram and is well suited for drug interaction investigations.
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