Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

pH Sensing by the calcium-sensing receptor.

Stephen J Quinn1, Mei Bai, Edward M Brown

  • 1Division of Endocrinology, Diabetes, and Hypertension, Department of Medicine, Brigham and Women's Hospital, and Harvard Medical School, Boston, Massachusetts 02115, USA. squinn@rics.bwh.harvard.edu

The Journal of Biological Chemistry
|June 18, 2004
PubMed
Summary

Extracellular pH significantly modulates the calcium-sensing receptor (CaR) activity, affecting its sensitivity to calcium and other agonists. This pH-dependent regulation is crucial for CaR function in various tissues, including the brain and kidney.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Study on Thermal Stability, Phase Transition Characteristics, and Pyrolysis Product Distributions of Long-Chain n-Alkanes (C12-C15).

Molecules (Basel, Switzerland)·2026
Same author

Stereoselective <i>C</i>-Aryl and <i>C</i>-Vinyl Glycosylation via Iron-Catalyzed Reductive Coupling.

Organic letters·2026
Same author

Excellent Ferroelectricity and Thermal Stability in Ce-Doped HfO<sub>2</sub> Thin Films via Oxygen Vacancy Modulation.

ACS applied materials & interfaces·2026
Same author

Temperature-responsive gelatin-based composite hydrogel pads with smart controlled phage release for intelligent antimicrobial packaging in chicken preservation.

Food research international (Ottawa, Ont.)·2026
Same author

Cox regression model for predicting surgical necrotizing enterocolitis based on abdominal radiographs radiomics features.

The British journal of radiology·2026
Same author

<i>Streptococcus bovismuti</i> sp. nov., a novel species isolated from yak milk.

International journal of systematic and evolutionary microbiology·2026

Area of Science:

  • Biochemistry
  • Cell Biology
  • Physiology

Background:

  • The calcium-sensing receptor (CaR) regulates serum calcium levels by sensing extracellular calcium (Ca(o)).
  • CaR is activated by polycationic molecules, suggesting electrostatic interactions are key to its function.
  • The influence of extracellular pH on CaR activity and its agonists has not been fully elucidated.

Purpose of the Study:

  • To investigate how extracellular pH modulates the activation of the calcium-sensing receptor (CaR) by various agonists.
  • To determine if changes in extracellular pH affect CaR sensitivity to calcium and other endogenous modulators.
  • To explore the potential role of CaR as a pH sensor in different physiological contexts.

Main Methods:

  • Utilized human embryonic kidney 293 cells expressing CaR.

Related Experiment Videos

  • Assessed CaR activation by calcium and other agonists across a range of extracellular pH values.
  • Investigated the impact of altered extracellular pH on CaR sensitivity and compared it with other G-protein-coupled receptors.

    • Main Results:

    • Extracellular pH significantly altered CaR activation by Ca(o) and other agonists.
    • Increased pH enhanced CaR sensitivity to agonists, while decreased pH reduced it.
    • Intracellular pH changes did not account for the observed effects; extracellular pH directly impacts CaR.

    Conclusions:

    • Extracellular pH is a critical modulator of calcium-sensing receptor (CaR) activity.
    • CaR exhibits altered sensitivity to agonists with deviations in pH, suggesting a direct interaction.
    • CaR may function as a sensor for pH and acid-base status in tissues like the stomach, kidney, bone, and brain.