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Solubilization of aggregates formed by expanded polyglutamine tract expression in cultured cells
Noriko Hazeki1, Ichiro Kanazawa
1Division of Neuroscience, Department of Neurology, CREST, Japan Science and Technology Corporation, Graduate School of Medicine, University of Tokyo, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|June 18, 2004
Abstract:
The expression of expanded polyglutamine in mammalian cells causes the formation of aggregates. For elucidation of the biochemical properties of the aggregates, isolation and solubilization of the aggregates are required. This chapter provides useful protocols for the solubilization of polyglutamine aggregates and further applications of protein analysis.
