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Effect of 5-fluorouracil on interleukin-2 expression
1Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore.
The effect of 5-fluorouracil on the expression of IL-2 gene and the production of IL-2 protein has been studied using spleenic lymphocytes of rats. The IL-2 messenger RNA has been quantitated by dot-blots and IL-2 has been assayed by Con A-blast assay. The results show that there is up to 3.75 fold increase in the production of IL-2 message in 5-fluorouracil treated lymphocytes over Con A treated controls. There is also a concomitant increase in IL-2 protein production upon 5-fluorouracil treatment in rat lymphocytes.
The effect of 5-fluorouracil on the expression of IL-2 gene and the production of IL-2 protein has been studied using spleenic lymphocytes of rats. The IL-2 messenger RNA has been quantitated by dot-blots and IL-2 has been assayed by Con A-blast assay. The results show that there is up to 3.75 fold increase in the production of IL-2 message in 5-fluorouracil treated lymphocytes over Con A treated controls. There is also a concomitant increase in IL-2 protein production upon 5-fluorouracil treatment in rat lymphocytes.