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Induction of MIP-1alpha in Kupffer cell by portal venous transfusion
Jong Kwon Park1, Kiho Cho, Jeremy Johnson
1Department of Surgery, College of Medicine, Dankook University, Cheonan, Chungnam 330-714, South Korea. hkpark@dankook.ac.kr
Introduction:
Previous studies have shown that portal venous transfusion (PVT) induces a state of immunosuppression, and Kupffer cells may be involved in the mechanism.
Objective:
This study was aimed to investigate the effect of PVT on Kupffer cell gene expression.
Materials And Methods:
Each BALB/C mouse was subjected to laparotomy and received one of five treatments: PVT, portal venous saline injection (PVS), inferior vena caval transfusion (IVCT), inferior vena caval saline injection (IVCS) or sham operation (S). The blood for PVT and IVCT was sampled from C57BL/6J mice. Kupffer cells were then isolated 1 or 24 h after each of the 5 treatments, for a total of 10 experimental groups (1-h PVT, PVS, IVCT, IVCS and S, and 24-h PVT, PVS, IVCT, IVCS and S) from BALB/C mice. To examine the effect of PVT on Kupffer cell gene expression, RT-PCR differential display was performed.
Results:
Increase in the expression of MIP-1alpha mRNA post PVT and IVCT was identified by differential display. PVT groups revealed higher levels of serum MIP-1alpha than any other groups.
Conclusion:
These results suggest that MIP-1alpha may be involved in a cascade of signaling events associated with the PVT-mediated immunologic modulation in Kupffer cells.
