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Carbohydrate changes in bladder carcinomas.

T F Orntoft1

  • 1University Department of Clinical Chemistry, Aarhus Kommunehospital, Denmark.

APMIS. Supplementum
|January 1, 1992
PubMed
Summary
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Cloning glycosyltransferases aids understanding of bladder tumor glycosylation. Analyzing urinary cells offers potential for cancer diagnosis and monitoring.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Glycosylation changes are observed in normal and malignant tissues, particularly in bladder tumors.
  • Specific enzymes like Gal alpha 1-3 and GalNac alpha 1-3 transferases show altered activity in bladder cancer.
  • Understanding gene expression and protein synthesis in different bladder tissue layers is crucial.

Purpose of the Study:

  • To investigate the molecular basis of altered glycosyltransferase activity in bladder tumors.
  • To explore the potential of urinary exfoliated cells for bladder cancer diagnosis and monitoring.
  • To study tumor-associated glycoconjugates and gene rearrangements in glycosyltransferase genes within urinary cells.

Main Methods:

  • Cloning of glycosyltransferases.

Related Experiment Videos

  • Analysis of enzyme activity (Gal alpha 1-3 and GalNac alpha 1-3 transferases).
  • Investigation of transcription and translation events in sorted bladder tissue layers.
  • Examination of urinary exfoliated cells for tumor markers.

    • Main Results:

    • The cloning of glycosyltransferases provides a breakthrough for studying glycosylation in normal and malignant tissues.
    • Bladder tumors exhibit specific changes in Gal alpha 1-3 and GalNac alpha 1-3 transferase activity requiring molecular explanation.
    • Urinary exfoliated cells present a viable source for detecting malignant cells.

    Conclusions:

    • Further research into glycosyltransferase gene rearrangements and urinary glycoconjugates is warranted for bladder cancer detection.
    • Studying cellular events in sorted bladder layers can elucidate molecular mechanisms of cancer.
    • The urine is a promising source for non-invasive bladder cancer diagnosis and monitoring.