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[Screening of antagonist peptide of BLyS from C7C phage display peptide library]
Qing Ji1, Feng-tian He, Rong-fen Li
1Department of Biochemistry & Molecular Biology, Third Military Medical University, Chongqing 400038, China.
Aim:
To screen an antagonist peptide of BLyS from C7C phage display peptide library.
Methods:
C7C phage display peptide library was screened with BLyS. Indirect ELISA, competitive ELISA and MTT colorimetry were used to identify positive phage clones.
Results:
After 3 rounds of screening, the gradual increase of the ratio of output to input and specific enrichment had been achieved. Two phage clones that could inhibit the BLyS activity were identified.
Conclusion:
Two phage display antagonist peptides of BLyS were obtained.
