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Updated: Aug 23, 2026

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles
Published on: November 13, 2015
[Cloning of human Nanog gene and its expression in COS-7L cells]
1Institute of Neuroscience, Fourth Military Medical University, Xi'an 710032, China. Liu123xia5798@sohu.com
Aim:
To clone human Nanog gene and express it in mammalian cells.
Methods:
LA-PCR was used to amplify human Nanog gene from genomic DNA of HEK 293 cells. The Nanog gene was inserted into the Flag-tagged pCMV vector by gene recombination technique. After being confirmed by sequencing, the pFlag-Nanog was transfected into COS-7L cells. Western blot and indirect immunofluorescent staining against the Flag tag were used to detect the expression of Nanog protein.
Results:
The sequence of the cloned Nanog was confirmed to be correct by sequence analysis. The COS-7L cells transfected with pFlag-Nanog could efficiently express Nanog protein.
Conclusion:
The cloning of human Nanog gene and the construction of its eukaryotic expression vector were successful. The study will lay the foundation for further research on the function of Nanog and its role in neural stem cells.
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