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Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Published on: October 15, 2013
[Pharyngitis due to Burkholderia cepacia. Person-to-person transmission]
M Fajardo Olivares1, J L Cordero Carrasco, A Beteta López
1Sección de Microbiología, Hospital Universitario Perpetuo Socorro, Damián Téllez Lafuente, s/n. 06010 Badajoz, Spain. mfolivares@eresmas.com
Abstract:
Burkholderia cepacia is a Gram-negative bacillus that is widely distributed in nature; it is isolated from the ground, water, plants and vegetables. Generally, it produces nosocomial infection due to contamination of disinfectants, medical equipment, prosthetic material and drugs, such as anesthetics or liquids used in urological irrigation. The most probable mechanism of transmission is through hospital material or through fomites among people after contact for several weeks or months. Recently, it has been considered as an important pathogen in immunocompromised patients, or in those with significant underlying diseases, such as chronic granulomastosis or cystic fibrosis. We present a case of pharyngitis due to B. cepacia and its transmission within a few days in two immunocompetent twin siblings without previous underlying diseases. The infection disappeared after specific treatment for this microorganism was started. We believe that samples should be taken from the pharynx and nasal pits in patients with acute upper respiratory tract processes that do not respond to empiric antibiotic treatment, before classifying them as viral infection without etiologic diagnosis.
Insights
Burkholderia cepacia can cause pharyngitis in healthy individuals. Rapid diagnosis and treatment are crucial for resolving this bacterial infection, even in immunocompetent patients.
Area of Science:
- Microbiology
- Infectious Diseases
- Clinical Medicine
Background:
- Burkholderia cepacia is a Gram-negative bacillus commonly found in natural environments.
- It is known to cause nosocomial infections, particularly in immunocompromised individuals.
- Transmission typically occurs via contaminated medical supplies or fomites.
Observation:
- A case of pharyngitis caused by Burkholderia cepacia is presented.
- The infection rapidly transmitted between two immunocompetent twin siblings.
- No underlying health conditions were noted in the affected individuals.
Findings:
- Burkholderia cepacia successfully treated with specific antimicrobial therapy.
- The infection resolved completely in both siblings.
- This highlights the potential for B. cepacia to cause acute infections in healthy individuals.
Implications:
- Suggests the need for pharyngeal and nasal sampling in non-responsive upper respiratory tract infections.
- Challenges the assumption of viral etiology in certain acute respiratory conditions.
- Emphasizes considering B. cepacia in the differential diagnosis of unexplained pharyngitis.
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