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Updated: Aug 23, 2026
A Rapid Automated Protocol for Muscle Fiber Population Analysis in Rat Muscle Cross Sections Using Myosin Heavy Chain Immunohistochemistry
Published on: March 28, 2017
A novel staining method for quantification and 3D visualisation of capillaries and muscle fibres
V Cebasek1, L Kubínová, S Ribaric
1Institute of Anatomy, Medical faculty, University of Ljubljana, Korytkova 2, 1000, Ljubljana, Slovenia. vita.cebasek@mf.uni-lj.si
Abstract:
The aim of this study was to introduce a combined fluorescent staining that clearly demonstrates capillaries and distinguishes them from the basal lamina of muscle fibres in skeletal muscle tissue. The triple staining with CD31, Griffonia (Bandeira) simplicifolia lectin (GSL I) and laminin efficiently distinguishes vascular endothelium from the basal lamina of skeletal muscle fibres in physiological and pathological conditions. The presented triple staining method has several advantages, which facilitate quantitative analysis of the capillary network, and its relation to individual muscle fibres.
