Related Experiment Video
Updated: Aug 23, 2026

The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development
Published on: October 5, 2016
A Pinus radiata AAA-ATPase, the expression of which increases with tree ageing
L Borja Diego1, María Berdasco, Mario F Fraga
1Laboratorio Fisiología Vegetal, Departamento BOS, Facultad de Biología, Universidad de Oviedo, C/ Catedrático Rodrigo Uría s/n. E-33071, Oviedo, Spain.
Abstract:
Age-dependent changes in gene expression profiles were studied in vegetative Pinus radiata buds by means of differential display. Among several candidate cDNAs, a 327 bp fragment that shows high homology with an Arabidopsis thaliana 20S proteasome ATPase designated RPT5a was found. Northern hybridization confirmed that the accumulation of this transcript increases with tree ageing, suggesting a possible role of this AAA-ATPase gene in development-related specific proteolysis.

