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Localization of alkaline phosphatase and proteins related to intercellular junctions in rat hepatoma cell line McA-RH
1Department of Anatomy, School of Allied Health Sciences, Kitasato University, Sagamihara, Kanagawa 228-8555, Japan. chida@ahs.kitasato-u.ac.jp
Abstract:
We investigated the localization of alkaline phosphatase (ALP) and three proteins related to intercellular junctions in the McA-RH 7777 rat hepatoma cell line to determine if the formation of junctions between adjacent McA-RH 7777 cells triggers translocation of ALP from cytoplasm to the plasma membrane. Contact between adjacent McA-RH 7777 cells promotes translocation of ALP from the Golgi area of the cytoplasm to the plasma membrane, and also promotes translocation of two proteins, E-cadherin and ZO-1, related to intercellular junctions, from cytoplasm to the plasma membrane.
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