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Updated: Aug 23, 2026

A High-content Assay for Monitoring AMPA Receptor Trafficking
Published on: January 28, 2019
Differential activity-dependent regulation of the lateral mobilities of AMPA and NMDA receptors
Laurent Groc1, Martin Heine, Laurent Cognet
1UMR 5091 CNRS-Université Bordeaux 2, Bordeaux 33077, France.
Abstract:
The basis for differences in activity-dependent trafficking of AMPA receptors (AMPARs) and NMDA receptors (NMDARs) remains unclear. Using single-molecule tracking, we found different lateral mobilities for AMPARs and NMDARs: changes in neuronal activity modified AMPAR but not NMDAR mobility, whereas protein kinase C activation modified both. Differences in mobility were mainly detected for extrasynaptic AMPARs, suggesting that receptor diffusion between synaptic and extrasynaptic domains is involved in plasticity processes.
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