Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

BRCA1 function mediates a TRAP/DRIP complex through direct interaction with TRAP220.

Osamu Wada1, Hajime Oishi, Ichiro Takada

  • 1Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-0034, Japan.

Oncogene
|June 23, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preoperative muscle health can predict distinct recovery patterns of patient-reported outcomes during 1 year after knee arthroplasty.

Clinical nutrition ESPEN·2026
Same author

Achieving normative quadriceps strength after total knee arthroplasty : associations with satisfaction, function, and a predictive nomogram.

The bone & joint journal·2026
Same author

Safety and effectiveness of routine postpartum bathing in a bathtub: A prospective study in Japan.

International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics·2026
Same author

Transition of Childhood Cancer Survivors into Adult Health Care: A Perspective from Adult Care.

Journal of adolescent and young adult oncology·2025
Same author

Transglutaminase 2 Stimulates Cell Proliferation and Modulates Transforming Growth Factor-Beta Signaling Pathway Independently of Epithelial-Mesenchymal Transition in Hepatocellular Carcinoma Cells.

International journal of molecular sciences·2025
Same author

The Role of ESS2/DGCR14: Is It an Essential Factor in Splicing and Transcription?

International journal of molecular sciences·2025

The BRCA1 tumor suppressor protein interacts with TRAP220, a key co-activator. This interaction is crucial for BRCA1

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • Breast cancer susceptibility gene 1 (BRCA1) is a critical tumor suppressor.
  • BRCA1 mutations are prevalent in hereditary breast and ovarian cancers.
  • BRCA1's C-terminal domain (BRCT) is vital for gene regulation and tumor suppression.

Purpose of the Study:

  • To investigate the interaction between the BRCA1 BRCT domain and co-regulator complexes.
  • To identify proteins associated with the BRCA1 BRCT domain.
  • To elucidate the role of TRAP220 in BRCA1-mediated tumor suppression.

Main Methods:

  • Biochemical isolation of BRCT-associated complexes from HeLa S3 nuclear extracts.
  • In vivo co-immunoprecipitation and in vitro glutathione S-transferase pull-down assays.

Related Experiment Videos

  • Transient expression assays and cell survival assays using antisense TRAP220.

    • Main Results:

    • TRAP220 was identified as a component of BRCT-associated complexes.
    • Direct interaction between BRCA1 BRCT and TRAP220 was confirmed in vitro and in vivo.
    • BRCA1 transactivation function and DNA damage-induced cell survival were dependent on TRAP220.

    Conclusions:

    • TRAP220 acts as a crucial co-activator for BRCA1.
    • The BRCA1-TRAP220 interaction plays a significant role in BRCA1-mediated tumor suppression.
    • TRAP220 complexes are important in the context of BRCA1 function in DNA repair and cell survival.