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Related Experiment Videos

An alkyl etherase in rat liver.

R A Peters, M Shorthouse

    The Journal of Physiology
    |November 1, 1975
    PubMed
    Summary

    Rat liver microsomes metabolize short-chain aliphatic ethers via an "etherase" enzyme. This process involves CoA acetylation, leading to citrate formation and entry into the citric acid cycle, without requiring tetrahydropteridine co-factors.

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    Area of Science:

    • Biochemistry
    • Enzymology
    • Metabolic pathways

    Background:

    • Diethyl ether is known to undergo partial metabolism in vivo.
    • Short-chain aliphatic ethers are substrates for metabolic processes.
    • Rat liver microsomes contain enzymes involved in ether metabolism.

    Purpose of the Study:

    • To investigate the enzymatic metabolism of short-chain aliphatic ethers in rat liver microsomes.
    • To elucidate the biochemical pathway and co-factors involved in ether metabolism.
    • To characterize the "etherase" activity and its products.

    Main Methods:

    • Incubation of rat liver microsomes with various short-chain aliphatic ethers.
    • Measurement of oxygen uptake in the presence of ethers.
    • Enzymatic assays to detect citrate and fluorocitrate formation.
    • Addition of coenzyme A (CoA), oxaloacetate, and synthase to identify metabolic intermediates.

    Main Results:

    • Rat liver microsomes exhibit oxygen uptake with diethyl ether, isopropyl ether, and n-butyl ether.
    • The "etherase" reaction is optimal at pH 7.2-7.4 and does not produce malondialdehyde.
    • Addition of CoA, oxaloacetate, and synthase to microsomes leads to citrate formation from diethyl ether, indicating CoA acetylation.
    • Methyl fluoroethyl ether is converted to fluorocitrate.
    • Tetrahydropteridine is not required as a co-factor, unlike in plasmalogen metabolism.

    Conclusions:

    • Rat liver microsomes possess an "etherase" enzyme capable of metabolizing short-chain aliphatic ethers.
    • Ether metabolism involves the acetylation of CoA, which then enters the citric acid cycle.
    • This pathway differs from plasmalogen metabolism in its co-factor requirements.

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