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Updated: Aug 23, 2026

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
Published on: January 5, 2017
Liquid-chromatography-coupled SAXS for accurate sizing of aggregating proteins
Elizabeth Mathew1, Ahmed Mirza, Nick Menhart
1Illinois Institute of Technology, 3101 South Dearborn Street, Chicago, IL 60616, USA.
Abstract:
Small-angle X-ray scattering and size-exclusion chromatography have been combined within a unified experimental set-up to obtain molecular size information. Besides providing simultaneous corroborative data bearing on the same question from two distinct experimental techniques, passing the samples over a gel filtration column immediately prior to illumination by X-rays provides both a more homogeneous sample and a continuous set of data as the concentration is extrapolated to zero. This greatly facilitates analysis of data from oligomerizing or aggregating proteins and increases the reliability of the results.
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