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Updated: Aug 23, 2026
Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
Published on: April 2, 2018
[Establishment of two-dimensional gel electrophoresis technical platform for proteome research]
Jianjun Liu1, Shisong Fang, Xiyi Li
1Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.
Objective:
To establish and optimize two-dimensional gel electrophoresis technique for proteomics.
Methods:
Immobiline pH gradients isoelectric focusing was used as 1D, vertical SDS-PAGE as 2D, some factors such as method and volume of loading sample, choice of gels, concentration of SDS gels, preset of electric program and parameters, protocol for staining the gels were improved.
Results And Conclusion:
Using the proper method described above, satisfactory 2DE maps of human embryonic lung fibroblast(HLF) and silkworm egg were obtained. 2DE technical platform for proteome research was set up.
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01:22Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
01:27SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...