Related Experiment Video
Updated: Aug 23, 2026
A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
Published on: February 6, 2018
[Effect of acrylonitrile on the spermatogenesis in mice]
Xiaoning Liu1, Wei Xiao, Zhenquan Wang
1School of Public Health, Lanzhou Medical College, Lanzhou 730000, China.
Objective:
To observe the effect of acrylonitrile on the spermatogenesis in mice.
Methods:
50 male pubertal mice and 25 male adult mice were exposed to acrylonitrile (1.25, 2.5, 5 mg/kg) by intraperitoneal injection for 5 days, and detected the DNA content and cell period of spermatogenic cells by flow cytometry after 35 days.
Results:
(1) The percentage of 1C cell in the exposed pubertal mice groups was lower than in the control group, respectively. The percentage of 1C cell in the exposed adult mice groups was lower than in the control group, respectively (P < 0.05). The percentage of apoptotic cell in pubertal and adult mice was higher than in the control group, respectively (P < 0.05). (2) The percentage of cells in S-phase in the exposed pubertal mice groups was higher than in the control group, respectively (P < 0.05), G0/G1 phase were lower(P < 0.05). The percentage of cells in G2/M phase in the exposed adult mice groups was higher than in the control group, respectively(P < 0.05), G0/G1 phase was lower(P < 0.05).
Conclusion:
There are restrained effects of acrylonitrile on the spermatogenesis in mice.
