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Updated: Jul 31, 2026
Pre-clinical Evaluation of Tyrosine Kinase Inhibitors for Treatment of Acute Leukemia
Published on: September 19, 2013
[Thymidine kinase gene mutation assay--comparison of microwell method and soft agar method]
Peiqiang Shuai1, Lishi Zhang, Yijing Wang
1School of Public Health, Sichuan University, Chengdu 610041, China.
Objective:
TK gene mutation assay was developed for a long time. Two methods are used in TK locus mutation assay, i.e. microwell method and soft agar method. Two methods were compared in this study so that to find out the better one to be used in China.
Methods:
In this study, two methods (microwell method and soft agar method) were used for detecting and evaluating the mutagenesis of three positive mutagens, i.e. MMS, EMS, and MMC, and a non-mutagen KCl.
Results:
Two methods was similar in results for three mutagens. For KCl, however, a negative result was showed in soft agar method while a positive result was showed in microwell method.
Conclusion:
According to the present study, it is recommended that the soft agar method should be popularized in China.
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