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Updated: Aug 23, 2026
Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
Published on: February 9, 2015
[Optimal culture of human bone marrow mesenchymal stem cells]
Yonggang Pang1, Wenxian Chen, Pengcheng Cui
1Department of Otorhinolaryngology, Tangdu Hospital of the Forth Military Medical University, Xi'an, Shaanxi, 710038, P. R. China.
Objective:
To optimize the culture conditions of the human bone marrow mesenchymal stem cells (hMSCs).
Methods:
The influence of the primary culture method, planting density, the time of the first medium changing, culture medium and serum concentration on growth of the hMSCs were analyzed.
Results:
When the other conditions were the same, the density gradient isolation was better than whole-marrow isolation; 2.5 x 10(5) cells/cm2 was the best planting density; the best first medium changing was the fifth day at primary culture, DMEM medium was better than alpha-MEM, serum C was the best of four serums compared, 10% was the suitable serum concentration. The hMSCs under the optimal conditions could expand over 15 passages, remaining their normal modality and differentiation potentials.
Conclusion:
The optimal culture condition of the hMSCs is established and it is a new investigation on application of hMSCs to tissue engineering.
