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Cellulose-based chromatography for cellooligosaccharide production.

Ozlem Akpinar1, Robert J McGorrin, Michael H Penner

  • 1Department of Food Science and Technology, Oregon State University, Corvallis, Oregon 97331-660, USA. akpinar99@yahoocom

Journal of Agricultural and Food Chemistry
|June 24, 2004
PubMed
Summary

Cellulose stationary phases effectively fractionate cellooligosaccharides (COS) using ethanol-water mobile phases. Higher degree of polymerization COS exhibit lower solubility and longer retention times, aiding in their separation and purification.

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Area of Science:

  • Analytical Chemistry
  • Food Science
  • Biochemistry

Background:

  • Cellooligosaccharides (COS) are gaining interest as functional, nondigestible oligosaccharides for food applications.
  • Efficient chromatographic methods are needed for the fractionation and purification of COS.

Purpose of the Study:

  • To explore the potential of cellulose stationary phases for chromatographic fractionation of COS.
  • To investigate the influence of mobile phase composition and temperature on COS separation.

Main Methods:

  • Utilized cellulose stationary phases (microcrystalline and fibrous) with ethanol-water mobile phases.
  • Investigated the solubility and elution order of COS based on their degree of polymerization (DP).
  • Analyzed the effect of temperature on retention volume.

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Main Results:

  • Cellulose stationary phases demonstrated effective fractionation of COS.
  • COS solubility and elution order correlated with DP; higher DP COS were less soluble and retained longer.
  • Increased temperature led to increased retention volume for all COS.

Conclusions:

  • Cellulose stationary phases are suitable for COS fractionation using ethanol-water mobile phases.
  • The method allows for the cleanup and fractionation of COS mixtures, such as those from cellulose hydrolysis.