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Generation of analytic plasma lipoprotein profiles using two prepacked superose 6B columns
L U Gerdes1, C Gerdes, I C Klausen
1Department of Internal Medicine and Cardiology A, Aarhus County Hospital, University of Aarhus, Denmark.
Clinica Chimica Acta; International Journal of Clinical Chemistry
|January 31, 1992
Summary
A new, affordable chromatographic system effectively analyzes plasma lipoprotein profiles in various lipid disorders. This method offers a cost-effective alternative to high-pressure liquid chromatography for lipoprotein analysis.
Area of Science:
- Clinical Chemistry
- Biochemistry
- Analytical Chemistry
Background:
- Accurate plasma lipoprotein profiling is crucial for diagnosing and managing dyslipidemias.
- Existing methods like high-pressure liquid chromatography (HPLC) can be expensive and require specialized equipment.
- There is a need for accessible and cost-effective analytical systems for lipoprotein analysis in clinical laboratories.
Purpose of the Study:
- To evaluate a simple, low-priced chromatographic system for generating plasma lipoprotein profiles.
- To assess the system's performance using plasma samples from individuals with various dyslipidemias and normolipidemic subjects.
- To determine if this system is a viable alternative to more expensive chromatographic techniques.
Main Methods:
- A simple, low-priced chromatographic system was employed to analyze total human plasma.
- Plasma samples from normolipidemic subjects and patients with specific familial lipid disorders were tested.
- Lipid measurements were performed on eluant fractions collected from the chromatographic columns.
Main Results:
- The chromatographic system successfully generated lipoprotein profiles from human plasma.
- The system demonstrated effectiveness across a range of lipid profiles, including those from patients with heterozygous familial hypercholesterolemia, hyper-alpha-lipoproteinemia, lipoprotein lipase deficiency, familial dysbetalipoproteinemia, and familial lecithin-cholesterol-acyl-transferase deficiency.
- The system provides a cost-effective alternative to high-pressure liquid chromatography (HPLC).
Conclusions:
- The evaluated chromatographic system is a practical and affordable tool for generating plasma lipoprotein profiles.
- It serves as a suitable alternative to more expensive HPLC systems, particularly for laboratories equipped for column chromatography.
- Integration with a microplate photometer and computer can further simplify lipid analysis in eluant fractions.