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Crystallization and preliminary crystallographic study of the functional form of the Bacillus thuringiensis
Panadda Boonserm1, Chanan Angsuthanasombat, Julien Lescar
1Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus, Nakornpathom 73170, Thailand. mbpbs@mahidol.ac.th
Abstract:
The 65 kDa functional form of the mosquito-larvicidal Cry4Aa-R235Q mutant toxin has been crystallized. The crystals belong to space group C222(1), with unit-cell parameters a = 91.2, b = 202.1, c = 98.7 A, and contain one molecule per asymmetric unit. The crystals diffract to approximately 2.9 A using synchrotron radiation and a complete native data set has been collected. The structure has been solved using a molecular-replacement method with the Cry4Ba toxin protein as a search model.

