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Updated: Jun 7, 2026

Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma
Published on: October 14, 2016
Osteosarcoma occurring in osteogenesis imperfecta
Shu Takahashi1, Kyoji Okada, Hiroyuki Nagasawa
1Department of Orthopedic Surgery, Section of Neuro and Locomotor Science, Akita University School of Medicine, Hondo 1-1-1, 010-8543 Akita, Japan. shu@med.akita-u.ac.jp
Abstract:
We describe a case history of a 24-year-old male with osteogenesis imperfecta (OI) who developed osteosarcoma of the left thigh. High-dose ifosfamide therapy caused marked tumor regression of multiple lung metastases. Immunohistochemically, the tumor cells were diffusely positive for the p53 protein. Mutation of the p53 gene was not detected by direct genomic sequencing of exons 4-8. The radiographic characteristics, including irregularly distributed osteolytic lesions and cortical discontinuity, should not be confused with hyperplastic callus formation, a benign process. A biopsy is critical to establish the differential diagnosis between osteosarcoma and common hyperplastic callus formation in OI; however, it must be applied with great care.
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