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Determination of microscopic acid-base parameters from NMR-pH titrations
Zoltán Szakács1, Márta Kraszni, Béla Noszál
1Department of Inorganic and Analytical Chemistry, Loránd Eötvös University, Pázmány Péter sétány 1/A, 1117 Budapest, Hungary.
Analytical and Bioanalytical Chemistry
|June 25, 2004
Abstract:
The theory and practice of proton microspeciation based on NMR-pH titrations are surveyed. Principles of bi-, tri-, tetra-, and n-protic microequilibrium systems are discussed. Evaluation methods are exemplified by case studies on bi- and tetraprotic biomolecules. Selection criteria and properties of 'reporter' NMR nuclei are described. Literature data on complete microspeciations of small ligands and site-specific basicity characterizations of peptides and proteins are critically reviewed.