Related Experiment Video
Updated: Aug 23, 2026

Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
Published on: February 15, 2018
Determination of equilibrium constants from chromatographic and electrophoretic measurements
1Faculty of Environmental Studies, University of Jan Evangelista Purkynĕ, Králova Výsina 7, 400 96 Ustí nod Labem, Czech Republic. janos@fzp.ujep.cz
Abstract:
Chemical interactions, such as acid-base, complex-forming, ion association and other equilibria, are widely exploited to improve the separation efficiency in liquid chromatography as well as in electrophoresis. On the other hand, these techniques can be advantageously used to study the chemical equilibria affecting the separations. If the equilibium is sufficiently fast in comparison with the separation process, then the retention characteristics in chromatography (retention factors) or the migration characteristics in electrophoresis (effective mobilities) may be expressed as functions of the composition of mobile phase or background electrolyte (BGE), respectively. Using a proper experimental arrangement, the dependencies of retention (migration) characteristics on the mobile phase (background electrolyte) composition can be measured and utilized to calculate the equilibrium constants for equlibria taking place in the mobile phase (background electrolyte). Although principles of these measurements have been known for a long time, only more recent studies utilizing HPLC and capillary electrophoretic techniques are reviewed in this paper.
Related Concept Videos
Chemical Equilibria: Systematic Approach to Equilibrium Calculations
The first step is to identify all the chemical reactions involved, The...
Chromatographic Methods: Terminology
The Equilibrium Binding Constant and Binding Strength
The Equilibrium Binding Constant and Binding Strength
Free Energy and Equilibrium
Recall that Q is the numerical value of the mass action expression...
Calculating the Equilibrium Constant
For example, gaseous nitrogen dioxide forms dinitrogen tetroxide according to this equation:

