Related Experiment Video
Updated: Aug 23, 2026
Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Published on: February 14, 2018
In vitro interactions of approved and novel drugs against Paecilomyces spp
Montserrat Ortoneda1, Javier Capilla, F Javier Pastor
1Unitat de Microbiologia, Facultat de Medicina i Ciencies de la Salut, Universitat Rovira i Virgili, Carrer Sant Llorenç, 21.43201 Reus, Spain.
Abstract:
We have evaluated the in vitro activity of 15 combinations of antifungal drugs (amphotericin B, itraconazole, voriconazole, albaconazole, ravuconazole, terbinafine, and micafungin) against four isolates of Paecilomyces variotii and three of P. lilacinus. The interaction of terbinafine with the four azoles was synergistic for 53% of the combinations, while the interactions of both amphotericin B and micafungin with the rest of antifungal agents were mainly indifferent.
Insights
This study explored antifungal drug combinations against Paecilomyces species. Terbinafine showed synergistic effects with azoles, while amphotericin B and micafungin interactions were mostly indifferent.
Area of Science:
- Mycology
- Pharmacology
- Infectious Diseases
Background:
- Paecilomyces species are emerging fungal pathogens.
- Effective antifungal treatments are crucial for managing Paecilomyces infections.
Purpose of the Study:
- To evaluate the in vitro activity of various antifungal drug combinations against Paecilomyces variotii and Paecilomyces lilacinus.
- To identify synergistic or indifferent interactions between different antifungal agents.
Main Methods:
- In vitro testing of 15 drug combinations.
- Antifungal drugs included amphotericin B, azoles (itraconazole, voriconazole, albaconazole, ravuconazole), terbinafine, and micafungin.
- Tested against four Paecilomyces variotii and three Paecilomyces lilacinus isolates.
Main Results:
- Terbinafine demonstrated synergistic interactions with 53% of the tested azole combinations.
- Amphotericin B and micafungin showed mainly indifferent interactions with other antifungal agents.
- Varied responses were observed across different drug combinations and Paecilomyces species.
Conclusions:
- Terbinafine combined with azoles presents a promising strategy for treating Paecilomyces infections.
- Amphotericin B and micafungin may have limited synergistic potential when combined with other antifungals in this context.
- Further in vivo studies are warranted to confirm these in vitro findings.
