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Updated: Aug 23, 2026

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
Published on: April 25, 2014
pncA mutations in pyrazinamide-resistant Mycobacterium tuberculosis isolates in Portugal
Isabel Portugal1, Luís Barreiro, José Moniz-Pereira
1Centro de Patogénese Molecular, Faculdade de Farmácia da Universidade de Lisboa, Rua Prof. Gama Pinto, 1649-003 Lisbon, Portugal. isabel.portugal@ff.ul.pt
Abstract:
The nucleotide sequences of the pncA genes within 55 multidrug-resistant pyrazinamide-resistant Mycobacterium tuberculosis clinical isolates were determined. Fifty-three out of the 55 isolates were pyrazinamidase (PZase) negative. Four strains contained a wild-type pncA gene, and PZase activity was undetectable in two of these strains. Seven of the 18 identified pncA mutations found have not been described in previous studies.
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