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Updated: Aug 23, 2026

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers
Published on: September 19, 2022
Enhancement of the enterocin CRL35 activity by a synthetic peptide derived from the NH2-terminal sequence
Lucila Saavedra1, Carlos Minahk, Aída P de Ruiz Holgado
1Centro de Referencia para Lactobacilos (CERELA-CONICET), S.M. de Tucumán (4000), Chacabuco 145, Tucumán, Argentina.
Abstract:
The enterocin CRL35 biosynthetic gene cluster was cloned and sequenced. The sequence was revealed to be highly identical to that of the mundticin KS gene cluster (S. Kawamoto, J. Shima, R. Sato, T. Eguchi, S. Ohmomo, J. Shibato, N. Horikoshi, K. Takeshita, and T. Sameshima, Appl. Environ. Microbiol. 68:3830-3840, 2002). Short synthetic peptides were designed based on the bacteriocin sequence and were evaluated in antimicrobial competitive assays. The peptide KYYGNGVSCNKKGCS produced an enhancement of enterocin CRL35 antimicrobial activity in a buffer system.

