The sequencing-based typing tool of dbMHC: typing highly polymorphic gene sequences
Related Experiment Video
Updated: Aug 23, 2026

Pyrosequencing: A Simple Method for Accurate Genotyping
Published on: January 8, 2008
Wolfgang Helmberg1, Raymond Dunivin, Michael Feolo
1National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Building 45, 8600 Rockville Pike, Bethesda, MD 20894, USA. helmberg@ncbi.nlm.nih.gov
Abstract:
The dbMHC resource (http://www.ncbi.nlm.nih.gov/mhc/sbt.cgi?cmd=main) at the National Center for Biotechnology Information (NCBI) has developed an online tool for evaluating the allelic composition of sequencing-based typing (SBT) results of cDNA or genomic sequences. Whether the samples are heterozygous, haploid or a combination of the two, they can be compared with two up-to-date databases of all known alleles of several human leukocyte antigen (HLA) and killer cell immunoglobulin-like receptor (KIR) loci. The results of the submission are returned as a table of potential allele hits, along with the respective base changes and an interactive sequence viewer for close examination of the alignment.
Related Concept Videos
Modern Molecular Taxonomy
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing
Evolutionary Relationships through Genome Comparisons
Applications of Molecular Taxonomy
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

