Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Paracrine Signaling

Paracrine Signaling

Paracrine signaling allows cells to communicate with their immediate neighbors via secretion of signaling molecules. Such a signal can only trigger a response in nearby target cells because the signal molecules degrade quickly or are inactivated if not taken up. Prominent examples of paracrine signaling include nitric oxide signaling in blood vessels, synaptic signaling of neurons, the blood clotting system, tissue repair/wound healing, and local allergic skin reactions. Nitric oxide as a...

Transgenic Organisms

Transgenic Organisms

Plant Tissue Culture

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

Mechanism of Angiogenesis

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Pre-mRNA splicing regulates cellular dedifferentiation via lipid metabolism in a cytokinin-dependent manner in Arabidopsis.

Plant physiology·2026

Same author

NIMA-related kinases redundantly regulate vegetative and reproductive development in Arabidopsis thaliana.

Plant & cell physiology·2026

Same author

Structural study of monomeric and dimeric photosystem I-LHCI supercomplexes from a bryophyte.

Communications biology·2026

Same author

Local peptide signalling induces stomatal closure under drought stress.

Nature communications·2025

Same author

LL3, a homolog of LONESOME HIGHWAY, regulates vascular cell proliferation in the root apical meristem.

Plant & cell physiology·2025

Same author

The response to thermospermine is fine-tuned by the balance between SAC51 and LHW family proteins in <i>Arabidopsis thaliana</i>.

Frontiers in plant science·2025

Search research articles

Related Experiment Video

Updated: May 12, 2026

In Utero Intra-cardiac Tomato-lectin Injections on Mouse Embryos to Gauge Renal Blood Flow

In Utero Intra-cardiac Tomato-lectin Injections on Mouse Embryos to Gauge Renal Blood Flow

Published on: February 4, 2015

A proteoglycan mediates inductive interaction during plant vascular development.

Hiroyasu Motose¹, Munetaka Sugiyama, Hiroo Fukuda

¹Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan. hmotose@ucdavis.edu

|June 25, 2004

Summary

This summary is machine-generated.

Researchers identified xylogen, a plant cell-wall protein, as a key inductive signal. Its polar secretion guides vascular differentiation, ensuring continuous plant vascular development.

More Related Videos

Coronary Progenitor Cells and Soluble Biomarkers in Cardiovascular Prognosis after Coronary Angioplasty

Coronary Progenitor Cells and Soluble Biomarkers in Cardiovascular Prognosis after Coronary Angioplasty

Published on: January 28, 2020

Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis

Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis

Published on: November 8, 2024

Related Experiment Videos

Last Updated: May 12, 2026

In Utero Intra-cardiac Tomato-lectin Injections on Mouse Embryos to Gauge Renal Blood Flow

In Utero Intra-cardiac Tomato-lectin Injections on Mouse Embryos to Gauge Renal Blood Flow

Published on: February 4, 2015

Coronary Progenitor Cells and Soluble Biomarkers in Cardiovascular Prognosis after Coronary Angioplasty

Coronary Progenitor Cells and Soluble Biomarkers in Cardiovascular Prognosis after Coronary Angioplasty

Published on: January 28, 2020

Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis

Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis

Published on: November 8, 2024

Area of Science:

Plant biology
Molecular biology
Developmental biology

Background:

Inductive cell-cell interactions are vital for cell fate determination.
The chemical signals driving these interactions in plants are not well understood.
Xylem differentiation relies on local inductive signals.

Purpose of the Study:

To purify and characterize xylogen, a potential inductive factor.
To investigate xylogen's role in plant vascular development in vitro and in planta.
To identify the molecule mediating inductive cell-cell interactions in plant tissue differentiation.

Main Methods:

Purification of xylogen and cloning of its complementary DNA.
Analysis of xylogen's molecular properties (hybrid proteoglycan).
Generation and analysis of Arabidopsis double knockout mutants for xylogen genes.

Main Results:

Xylogen was purified and its cDNA cloned, revealing a hybrid protein structure.
Xylogen accumulates in meristem, procambium, and xylem, with polar localization in differentiating tracheary elements.
Arabidopsis mutants lacking xylogen genes exhibited vascular defects, including discontinuous veins and simplified venation.

Conclusions:

Xylogen acts as a polar secreted factor mediating inductive cell-cell interactions.
This interaction is crucial for directing vascular differentiation and ensuring continuous vascular development in plants.
Xylogen identifies a key molecule in plant tissue differentiation processes.