Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Developing novel activity-based fluorescent probes that target different classes of proteases.

Qing Zhu1, Aparna Girish, Souvik Chattopadhaya

  • 1Department of Chemistry, National University of Singapore, Singapore.

Chemical Communications (Cambridge, England)
|June 25, 2004
PubMed
Summary

Researchers developed new activity-based probes targeting protease sub-classes by substrate specificity. This novel approach was successfully demonstrated, offering a new tool for protease research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In Situ Monitoring of Heterogeneous Hydrosilylation Reactions Using Infrared and Raman Spectroscopy: Normalization Using Phase-Specific Internal Standards.

Applied spectroscopy·2019
Same author

Glycosidic Acids with Unusual Aglycone Units from Convolvulus arvensis.

Journal of natural products·2019
Same author

[Research on the Whole-process Cloud Monitoring Mode of <i>in Vitro</i> Diagnostic Medical Devices Adverse Events].

Zhongguo yi liao qi xie za zhi = Chinese journal of medical instrumentation·2019
Same author

FHL3 negatively regulates the differentiation of skeletal muscle satellite cells in chicken.

3 Biotech·2019
Same author

Silver nanoparticles selectively induce human oncogenic γ-herpesvirus-related cancer cell death through reactivating viral lytic replication.

Cell death & disease·2019
Same author

Palmatine plays a role in sedation and hypnosis by increasing 5-hydroxytryptamine.

Cellular and molecular biology (Noisy-le-Grand, France)·2019

Area of Science:

  • Biochemistry
  • Chemical Biology
  • Enzymology

Background:

  • Proteases are crucial enzymes involved in numerous biological processes.
  • Current activity-based probes often target protease mechanisms, limiting their specificity.
  • A need exists for probes that differentiate protease sub-classes based on substrate recognition.

Purpose of the Study:

  • To design and synthesize novel activity-based probes targeting protease sub-classes.
  • To develop probes based on substrate specificities rather than enzymatic mechanisms.
  • To demonstrate the feasibility of this substrate-specificity-based approach.

Main Methods:

  • Design of novel chemical structures for activity-based probes.
  • Synthesis of the designed probes.

Related Experiment Videos

  • Validation of probe activity and specificity against different protease sub-classes.
  • Utilizing representative members of protease sub-classes for demonstration.

    • Main Results:

    • Successful design and synthesis of a group of novel activity-based probes.
    • Demonstration that these probes effectively target protease sub-classes based on substrate specificities.
    • Proof-of-concept validation using representative protease examples.

    Conclusions:

    • The developed activity-based probes represent a novel strategy for targeting protease sub-classes.
    • Substrate specificity is a viable basis for designing targeted protease probes.
    • This approach offers enhanced specificity and potential for new biological insights.