Jove
Visualize
Contact Us

Related Experiment Videos

A simple method to adjust cooling rates for supercooling point determination.

M A Carrillo1, N Kaliyan, C A Cannon

  • 1Department of Entomology, University of Minnesota, Saint Paul, MN 55108, USA.

Cryo Letters
|June 25, 2004
PubMed
Summary

This study introduces a simple, economical method using polystyrene cubes to achieve constant cooling rates for insect supercooling point (SCP) determination. The technique accurately measures SCPs across different insect developmental stages.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gas sensing in nematodes.

Molecular neurobiology·2014
Same author

Mortality of Indianmeal moth (Lepidoptera: Pyralidae) populations under fluctuating low temperatures: model development and validation.

Environmental entomology·2008
Same author

Relationship between supercooling point and mortality at low temperatures in Indianmeal moth (Lepidoptera: Pyralidae).

Journal of economic entomology·2005
Same author

Cold hardiness of Habrobracon hebetor (Say) (Hymenoptera: Braconidae), a parasitoid of pyralid moths.

Journal of insect physiology·2005
Same author

Seepage from deep bedded and poultry litter systems.

Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes·2000
Same author

Factors influencing cellular immune responses to feline immunodeficiency virus induced by DNA vaccination.

Vaccine·1999
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Thermal Biology
  • Insect Physiology
  • Cryobiology

Background:

  • Accurate determination of insect supercooling points (SCPs) is crucial for understanding cold hardiness.
  • Existing methods for controlling cooling rates can be complex or inflexible.

Purpose of the Study:

  • To develop and validate a simple, cost-effective method for achieving predetermined constant cooling rates for insect SCP determination.
  • To assess the applicability of this method across different insect developmental stages.

Main Methods:

  • Designed polystyrene cubes of varying sizes based on transient heat transfer equations to achieve specific cooling rates.
  • Measured cooling rates at the geometric center of the cubes under constant surrounding temperatures.
  • Determined supercooling points of Tribolium castaneum at different developmental stages using the developed method.

Related Experiment Videos

Main Results:

  • Polystyrene cubes of 0.29m and 0.19m yielded constant cooling rates of approximately -0.5°C/min and -1°C/min, respectively.
  • Observed cooling rates closely matched predictions from heat transfer equations.
  • Measured SCPs for Tribolium castaneum were consistent with published data.

Conclusions:

  • The polystyrene cube method provides a reliable and precise way to control cooling rates for SCP determination.
  • This economical, flexible method allows for simultaneous SCP measurements, advancing insect cold hardiness research.