Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Microtubule's conformational cap.

D Chrétien¹, I Jáinosi, J C Taveau

¹Laboratoire des Protéines Complexes, Université de Tours, 2 bis Boulevard Tonnellé, F-37032 Tours, France. denis.chretien@univ-rennes1.fr

Cell Structure and Function

|June 26, 2004

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Pyruvate carboxylase deficiency: An underestimated cause of lactic acidosis.

Molecular genetics and metabolism reports·2017

Same author

How to Measure Load-Dependent Kinetics of Individual Motor Molecules Without a Force-Clamp.

Methods in enzymology·2017

Same author

How to Measure Separations and Angles Between Intramolecular Fluorescent Markers.

Methods in enzymology·2016

Same author

A multi-center comparison of diagnostic methods for the biochemical evaluation of suspected mitochondrial disorders.

Mitochondrion·2012

Same author

Normal oxidative phosphorylation in intestinal smooth muscle of childhood chronic intestinal pseudo-obstruction.

Neurogastroenterology and motility·2010

Same author

Calcium signalling-dependent mitochondrial dysfunction and bioenergetics regulation in respiratory chain Complex II deficiency.

Cell death and differentiation·2010

Same journal

Jaw1 controls the granule abundance at a distinct ER compartment of pancreatic acinar cells.

Cell structure and function·2026

Same journal

Looking back on 50 years of cell biology in Japan.

Cell structure and function·2026

Same journal

CCL5 enhances invasion of squamous cell carcinoma via syndecan-1-dependent ERK signaling.

Cell structure and function·2026

Same journal

Metformin attenuates cuprizone-induced mitochondrial dysfunction and senescence-associated changes in primary neuronal cells.

Cell structure and function·2026

Same journal

Loss of macroautophagy results in abnormality of the endoplasmic reticulum in insulin-secreting MIN6 cells.

Cell structure and function·2026

Same journal

Changes in nuclear morphology and size in mammalian temperature-sensitive mutants.

Cell structure and function·2026

See all related articles

Microtubule elongation mechanisms are clarified by revealing distinct lattice and wall stabilities. Elastic properties explain differences between the tubulin sheet and microtubule wall during growth.

Area of Science:

Biochemistry
Cell Biology
Biophysics

Background:

Microtubule dynamics are crucial for cellular processes.
The "GTP-cap" model explains microtubule elongation.
Unstable microtubule lattice elongation mechanisms require further elucidation.

Purpose of the Study:

To investigate the molecular mechanisms behind microtubule lattice elongation.
To challenge the conventional "GTP-cap" model of microtubule dynamics.
To explore the role of elastic properties in microtubule stability.

Main Methods:

Theoretical analysis of microtubule lattice elasticity.
Modeling of tubulin sheet and microtubule wall stability.
Comparison of theoretical predictions with existing experimental data.

Related Experiment Videos

Main Results:

The elastic properties of the microtubule lattice create differential stability between the elongating tubulin sheet and the completed microtubule wall.
This difference in stability provides a new framework for understanding microtubule elongation.
The proposed mechanism offers an alternative to the traditional "GTP-cap" model.

Conclusions:

Elastic properties are key determinants of microtubule lattice stability and elongation.
The study reframes the understanding of microtubule dynamics beyond the "GTP-cap" model.
Further research should explore the experimental validation of these findings.