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Related Concept Videos

Fermentation01:29

Fermentation

Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
Bioreactor Controls-I01:28

Bioreactor Controls-I

Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Scale-Up Processes01:14

Scale-Up Processes

The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
Production of Alcohol01:27

Production of Alcohol

Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...

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Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
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Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

A multi-scale study of industrial fermentation processes and their optimization.

Siliang Zhang1, Ju Chu, Yingping Zhuang

  • 1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. siliangz@163.net

Advances in Biochemical Engineering/Biotechnology
|June 26, 2004
PubMed
Summary

Computer simulations addressed multi-scale industrial fermentation challenges across molecular, cellular, and engineering levels. Novel bioreactor systems enabled significant productivity improvements in key bioprocesses.

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Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Industrial Microbiology

Background:

  • Industrial fermentation processes involve complex multi-scale challenges.
  • Optimizing these processes requires integrating insights from molecular genetics, cellular metabolism, and reactor engineering.

Purpose of the Study:

  • To discuss problems in multi-scale industrial fermentation.
  • To present an optimization methodology and scale-up technique for bioprocesses.
  • To introduce a novel bioreactor system for mass flux monitoring.

Main Methods:

  • Computer simulations were used to model fermentation processes at molecular, cellular, and reactor engineering scales.
  • Metabolic flux analysis and control were employed for bioreaction engineering.
  • A novel bioreactor system was designed and applied for mass flux monitoring.

Main Results:

  • Inter-scale observation and operation were identified as crucial for bioprocess optimization.
  • The developed methodology and scale-up technique were generalized through investigation of two typical fermentation processes.
  • The novel bioreactor system successfully optimized and scaled up industrial fermentation for various products, including pharmaceuticals and recombinant proteins.

Conclusions:

  • Substantial improvements in industrial fermentation productivity were achieved.
  • The integration of multi-scale data and advanced monitoring is key to enhancing bioprocess efficiency.
  • The developed approach offers a generalized solution for optimizing and scaling up diverse fermentation processes.