Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Improved method for electroporation of Staphylococcus aureus.

S Schenk1, R A Laddaga

  • 1Department of Biological Sciences, Bowling Green State University, Ohio 43403.

FEMS Microbiology Letters
|July 1, 1992
PubMed
Summary

We developed a new electroporation method for Staphylococcus aureus, achieving a 530-fold increase in transformation efficiency. This breakthrough utilizes a modified B2 medium and 0.1-cm cuvettes for enhanced plasmid DNA delivery.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Temporal overexpression of SIRT1 in skeletal muscle of adult mice does not improve insulin sensitivity or markers of mitochondrial biogenesis.

Acta physiologica (Oxford, England)·2017
Same author

Relatively low endogenous fatty acid mobilization and uptake helps preserve insulin sensitivity in obese women.

International journal of obesity (2005)·2014
Same author

A genetic deletion of the serotonin transporter greatly enhances the reinforcing properties of MDMA in rats.

Molecular psychiatry·2013
Same author

Skeletal muscle-specific overexpression of SIRT1 does not enhance whole-body energy expenditure or insulin sensitivity in young mice.

Diabetologia·2013
Same author

[Indolent volar swelling of the finger].

Der Radiologe·2012
Same author

Regulation of plasma ceramide levels with fatty acid oversupply: evidence that the liver detects and secretes de novo synthesised ceramide.

Diabetologia·2012

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Electroporation is a key method for introducing plasmid DNA into bacteria.
  • Previous methods for Staphylococcus aureus electroporation had limited efficiency.

Purpose of the Study:

  • To significantly improve the efficiency of plasmid DNA electroporation into Staphylococcus aureus.
  • To identify key factors contributing to enhanced transformation efficiency.

Main Methods:

  • Development of a modified growth medium (B2 medium).
  • Optimization of electroporation parameters, including cuvette size.
  • Testing transformation efficiency with various plasmids and bacterial strains.

Main Results:

Related Experiment Videos

  • Achieved a transformation efficiency of 4.0 x 10(8) transformants/µg of pSK265 DNA, a 530-fold improvement.
  • Identified modified medium and 0.1-cm cuvettes as primary factors for efficiency.
  • Demonstrated significantly higher efficiency for pSK265 compared to other plasmids (pI258, pMH109, pSK270).

    • Conclusions:

    • The developed electroporation protocol offers a substantial advancement for genetic manipulation of Staphylococcus aureus.
    • The method is highly effective for pSK265 plasmid transformation but shows reduced efficiency with other plasmids and staphylococcal species.