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Antiphospholipid antibodies: do we still need to perform anticardiolipin ELISA assays?

P de Moerloose1, G Reber

  • 1Division of Angiology and Hemostasis, University Hospital, Geneva, Switzerland. philippe.demoerloose@hcuge.ch

Journal of Thrombosis and Haemostasis : JTH
|June 29, 2004
PubMed
Abstract

No abstract available in PubMed .

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.