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Updated: Aug 23, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
The stress reaction and its molecular events: splicing variants
Li-Ling Tang1, Yuan-Liang Wang, Cai-Xin Sun
1Key Laboratory of Biomechanics and Tissue Engineering of Ministry of Education, Bio-engineering College, Chongqing University, Chongqing 400044, PR China. huayue@cta.cq.cn
Abstract:
The growth of cells and tissues is regulated by stress. When body is injured, it manifests a large spectrum of metabolic, endocrine, and immune alterations, which is named stress reaction. Among them, the production of growth factors may play a critical role. For osteoblasts and myoblasts, IGF-I has been shown to be involved in the process of cells in response to overloads. There are two splicing forms, one is IGF-Ea, the other is the IGF-IEb in the rodents and corresponds to IGF-IEc in humans. The latter is markedly up-regulated in response to overloads. Therefore, it has been named mechanogrowth factor. The link between the mechanical stimulus and the gene expression represents a new and important area in cell science. Understanding the process of splicing in IGF-I helps one to investigate the mechanotransduction of cells in response to mechanical stimulation at molecular level.
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