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Updated: Aug 23, 2026

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Published on: October 16, 2017
On the separation, detection and quantification of pectin derived oligosaccharides by capillary electrophoresis
Anna Ström1, Martin A K Williams
1Unilever R&D Colworth, Sharnbrook, MK44 1LQ Bedford, UK.
Abstract:
Having previously reported that capillary electrophoresis can be used as a tool for the analysis of partially methyl-esterified oligogalacturonides we now describe a method that improves the resolution of individual oligomers, and detail a more rigorous quantification scheme that uses an internal standard and takes into account the relative molecular absorbance of different partially methyl-esterified species. The internal consistency of the method is subsequently demonstrated by performing the quantification of an endo-polygalacturonase pectin digest before and after de-methylation of the resultant oligomers.
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